zero-d software package (Molecular Dynamics Inc)
90
Structured Review
Molecular Dynamics Inc
zero-d software package
Zero D Software Package, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zero-d+software+package/zero+d+software+package/pm11174852-66-11-14
Average 90 stars, based on 1 article reviews
Zero D Software Package, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zero-d+software+package/zero+d+software+package/pm11174852-66-11-14
Average 90 stars, based on 1 article reviews
zero-d software package - by Bioz Stars,
2026-10
90/100 stars
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Autoradiography:Article Title: 3,3'4,4'-Tetrachlorobiphenyl exhibits antiestrogenic and antitumorigenic activity in the rodent uterus and mammary cells and in human breast cancer cells. Article Snippet: Protein–DNA complexes were resolved on a 5–6% polyacrylamide gel (acrylamide:bisacrylamide ratio, 30:0.8) and run in 13 TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 8.3) at 110 V. Protein–DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen induces adenosine deaminase gene expression in MCF-7 human breast cancer cells: role of estrogen receptor-Sp1 interactions. Article Snippet: After amplification, products were separated on a 7% (wt/vol) polyacrylamide gel, visualized by autoradiography using Kodak XAR film (Eastman Kodak Co., Rochester, NY), and quantitated by densitometry using the Article Title: Transcriptional activation of heat shock protein 27 gene expression by 17beta-estradiol and modulation by antiestrogens and aryl hydrocarbon receptor agonists. Article Snippet: Band intensities were also quantitated by densitometry using the Molecular Dynamics Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: 32P-Labeled DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of c-fos protooncogene by 17beta-estradiol: mechanism of aryl hydrocarbon receptor-mediated inhibition. Article Snippet: [32P]DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: 3',4'-dimethoxyflavone as an aryl hydrocarbon receptor antagonist in human breast cancer cells. Article Snippet: Protein-DNA complexes were resolved on a 5– 6% polyacrylamide gel (acrylamide:bisacrylamide ration, 30:0.8) and run in 1X TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 3.8) at 110 V. Bound complexes were visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen-induced retinoic acid receptor alpha 1 gene expression: role of estrogen receptor-Sp1 complex. Article Snippet: The following procedure was used for ER-enhanced Sp1 binding studies: 1) 200–800 fmol pure hER protein in 1 3 binding buffer containing 40 mM E2 and BSA was incubated for 15 min at 4 C; 2) 1–5 ng Sp1 protein was added to the mixture and incubated on ice for 5 min; 3) 32Plabeled oligonucleotides (50,000 cpm) were added to the reaction mixture in the presence of 0.5 mg poly d(I-C), and the mixture was incubated for an additional 15 min at 25 C. Samples were loaded onto a 5% polyacrylamide gel (acrylamide-bisacrylamide ratio, 30:0.8) and run in 1 3 TBE buffer (0.09 M Tris, 0.09 boric acid, and 2 mM EDTA, pH 8.3) at 110 V. Protein-DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: Quantitation of band intensities used a Software:Article Title: 3,3'4,4'-Tetrachlorobiphenyl exhibits antiestrogenic and antitumorigenic activity in the rodent uterus and mammary cells and in human breast cancer cells. Article Snippet: Protein–DNA complexes were resolved on a 5–6% polyacrylamide gel (acrylamide:bisacrylamide ratio, 30:0.8) and run in 13 TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 8.3) at 110 V. Protein–DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen induces adenosine deaminase gene expression in MCF-7 human breast cancer cells: role of estrogen receptor-Sp1 interactions. Article Snippet: After amplification, products were separated on a 7% (wt/vol) polyacrylamide gel, visualized by autoradiography using Kodak XAR film (Eastman Kodak Co., Rochester, NY), and quantitated by densitometry using the Article Title: Transcriptional activation of heat shock protein 27 gene expression by 17beta-estradiol and modulation by antiestrogens and aryl hydrocarbon receptor agonists. Article Snippet: Band intensities were also quantitated by densitometry using the Molecular Dynamics Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: 32P-Labeled DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of c-fos protooncogene by 17beta-estradiol: mechanism of aryl hydrocarbon receptor-mediated inhibition. Article Snippet: [32P]DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: 3',4'-dimethoxyflavone as an aryl hydrocarbon receptor antagonist in human breast cancer cells. Article Snippet: Protein-DNA complexes were resolved on a 5– 6% polyacrylamide gel (acrylamide:bisacrylamide ration, 30:0.8) and run in 1X TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 3.8) at 110 V. Bound complexes were visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen-induced retinoic acid receptor alpha 1 gene expression: role of estrogen receptor-Sp1 complex. Article Snippet: The following procedure was used for ER-enhanced Sp1 binding studies: 1) 200–800 fmol pure hER protein in 1 3 binding buffer containing 40 mM E2 and BSA was incubated for 15 min at 4 C; 2) 1–5 ng Sp1 protein was added to the mixture and incubated on ice for 5 min; 3) 32Plabeled oligonucleotides (50,000 cpm) were added to the reaction mixture in the presence of 0.5 mg poly d(I-C), and the mixture was incubated for an additional 15 min at 25 C. Samples were loaded onto a 5% polyacrylamide gel (acrylamide-bisacrylamide ratio, 30:0.8) and run in 1 3 TBE buffer (0.09 M Tris, 0.09 boric acid, and 2 mM EDTA, pH 8.3) at 110 V. Protein-DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: Quantitation of band intensities used a Quantitation Assay:Article Title: 3,3'4,4'-Tetrachlorobiphenyl exhibits antiestrogenic and antitumorigenic activity in the rodent uterus and mammary cells and in human breast cancer cells. Article Snippet: Protein–DNA complexes were resolved on a 5–6% polyacrylamide gel (acrylamide:bisacrylamide ratio, 30:0.8) and run in 13 TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 8.3) at 110 V. Protein–DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen induces adenosine deaminase gene expression in MCF-7 human breast cancer cells: role of estrogen receptor-Sp1 interactions. Article Snippet: After amplification, products were separated on a 7% (wt/vol) polyacrylamide gel, visualized by autoradiography using Kodak XAR film (Eastman Kodak Co., Rochester, NY), and quantitated by densitometry using the Article Title: Transcriptional activation of heat shock protein 27 gene expression by 17beta-estradiol and modulation by antiestrogens and aryl hydrocarbon receptor agonists. Article Snippet: Band intensities were also quantitated by densitometry using the Molecular Dynamics Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: 32P-Labeled DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of c-fos protooncogene by 17beta-estradiol: mechanism of aryl hydrocarbon receptor-mediated inhibition. Article Snippet: [32P]DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: 3',4'-dimethoxyflavone as an aryl hydrocarbon receptor antagonist in human breast cancer cells. Article Snippet: Protein-DNA complexes were resolved on a 5– 6% polyacrylamide gel (acrylamide:bisacrylamide ration, 30:0.8) and run in 1X TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 3.8) at 110 V. Bound complexes were visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen-induced retinoic acid receptor alpha 1 gene expression: role of estrogen receptor-Sp1 complex. Article Snippet: The following procedure was used for ER-enhanced Sp1 binding studies: 1) 200–800 fmol pure hER protein in 1 3 binding buffer containing 40 mM E2 and BSA was incubated for 15 min at 4 C; 2) 1–5 ng Sp1 protein was added to the mixture and incubated on ice for 5 min; 3) 32Plabeled oligonucleotides (50,000 cpm) were added to the reaction mixture in the presence of 0.5 mg poly d(I-C), and the mixture was incubated for an additional 15 min at 25 C. Samples were loaded onto a 5% polyacrylamide gel (acrylamide-bisacrylamide ratio, 30:0.8) and run in 1 3 TBE buffer (0.09 M Tris, 0.09 boric acid, and 2 mM EDTA, pH 8.3) at 110 V. Protein-DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: Quantitation of band intensities used a Binding Assay:Article Title: 3,3'4,4'-Tetrachlorobiphenyl exhibits antiestrogenic and antitumorigenic activity in the rodent uterus and mammary cells and in human breast cancer cells. Article Snippet: Protein–DNA complexes were resolved on a 5–6% polyacrylamide gel (acrylamide:bisacrylamide ratio, 30:0.8) and run in 13 TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 8.3) at 110 V. Protein–DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen induces adenosine deaminase gene expression in MCF-7 human breast cancer cells: role of estrogen receptor-Sp1 interactions. Article Snippet: After amplification, products were separated on a 7% (wt/vol) polyacrylamide gel, visualized by autoradiography using Kodak XAR film (Eastman Kodak Co., Rochester, NY), and quantitated by densitometry using the Article Title: Transcriptional activation of heat shock protein 27 gene expression by 17beta-estradiol and modulation by antiestrogens and aryl hydrocarbon receptor agonists. Article Snippet: Band intensities were also quantitated by densitometry using the Molecular Dynamics Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: 32P-Labeled DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of c-fos protooncogene by 17beta-estradiol: mechanism of aryl hydrocarbon receptor-mediated inhibition. Article Snippet: [32P]DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: 3',4'-dimethoxyflavone as an aryl hydrocarbon receptor antagonist in human breast cancer cells. Article Snippet: Protein-DNA complexes were resolved on a 5– 6% polyacrylamide gel (acrylamide:bisacrylamide ration, 30:0.8) and run in 1X TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 3.8) at 110 V. Bound complexes were visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen-induced retinoic acid receptor alpha 1 gene expression: role of estrogen receptor-Sp1 complex. Article Snippet: The following procedure was used for ER-enhanced Sp1 binding studies: 1) 200–800 fmol pure hER protein in 1 3 binding buffer containing 40 mM E2 and BSA was incubated for 15 min at 4 C; 2) 1–5 ng Sp1 protein was added to the mixture and incubated on ice for 5 min; 3) 32Plabeled oligonucleotides (50,000 cpm) were added to the reaction mixture in the presence of 0.5 mg poly d(I-C), and the mixture was incubated for an additional 15 min at 25 C. Samples were loaded onto a 5% polyacrylamide gel (acrylamide-bisacrylamide ratio, 30:0.8) and run in 1 3 TBE buffer (0.09 M Tris, 0.09 boric acid, and 2 mM EDTA, pH 8.3) at 110 V. Protein-DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: Quantitation of band intensities used a Amplification:Article Title: 3,3'4,4'-Tetrachlorobiphenyl exhibits antiestrogenic and antitumorigenic activity in the rodent uterus and mammary cells and in human breast cancer cells. Article Snippet: Protein–DNA complexes were resolved on a 5–6% polyacrylamide gel (acrylamide:bisacrylamide ratio, 30:0.8) and run in 13 TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 8.3) at 110 V. Protein–DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen induces adenosine deaminase gene expression in MCF-7 human breast cancer cells: role of estrogen receptor-Sp1 interactions. Article Snippet: After amplification, products were separated on a 7% (wt/vol) polyacrylamide gel, visualized by autoradiography using Kodak XAR film (Eastman Kodak Co., Rochester, NY), and quantitated by densitometry using the Article Title: Transcriptional activation of heat shock protein 27 gene expression by 17beta-estradiol and modulation by antiestrogens and aryl hydrocarbon receptor agonists. Article Snippet: Band intensities were also quantitated by densitometry using the Molecular Dynamics Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: 32P-Labeled DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of c-fos protooncogene by 17beta-estradiol: mechanism of aryl hydrocarbon receptor-mediated inhibition. Article Snippet: [32P]DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: 3',4'-dimethoxyflavone as an aryl hydrocarbon receptor antagonist in human breast cancer cells. Article Snippet: Protein-DNA complexes were resolved on a 5– 6% polyacrylamide gel (acrylamide:bisacrylamide ration, 30:0.8) and run in 1X TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 3.8) at 110 V. Bound complexes were visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen-induced retinoic acid receptor alpha 1 gene expression: role of estrogen receptor-Sp1 complex. Article Snippet: The following procedure was used for ER-enhanced Sp1 binding studies: 1) 200–800 fmol pure hER protein in 1 3 binding buffer containing 40 mM E2 and BSA was incubated for 15 min at 4 C; 2) 1–5 ng Sp1 protein was added to the mixture and incubated on ice for 5 min; 3) 32Plabeled oligonucleotides (50,000 cpm) were added to the reaction mixture in the presence of 0.5 mg poly d(I-C), and the mixture was incubated for an additional 15 min at 25 C. Samples were loaded onto a 5% polyacrylamide gel (acrylamide-bisacrylamide ratio, 30:0.8) and run in 1 3 TBE buffer (0.09 M Tris, 0.09 boric acid, and 2 mM EDTA, pH 8.3) at 110 V. Protein-DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: Quantitation of band intensities used a Incubation:Article Title: 3,3'4,4'-Tetrachlorobiphenyl exhibits antiestrogenic and antitumorigenic activity in the rodent uterus and mammary cells and in human breast cancer cells. Article Snippet: Protein–DNA complexes were resolved on a 5–6% polyacrylamide gel (acrylamide:bisacrylamide ratio, 30:0.8) and run in 13 TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 8.3) at 110 V. Protein–DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen induces adenosine deaminase gene expression in MCF-7 human breast cancer cells: role of estrogen receptor-Sp1 interactions. Article Snippet: After amplification, products were separated on a 7% (wt/vol) polyacrylamide gel, visualized by autoradiography using Kodak XAR film (Eastman Kodak Co., Rochester, NY), and quantitated by densitometry using the Article Title: Transcriptional activation of heat shock protein 27 gene expression by 17beta-estradiol and modulation by antiestrogens and aryl hydrocarbon receptor agonists. Article Snippet: Band intensities were also quantitated by densitometry using the Molecular Dynamics Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: 32P-Labeled DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of c-fos protooncogene by 17beta-estradiol: mechanism of aryl hydrocarbon receptor-mediated inhibition. Article Snippet: [32P]DNA and DNA-protein bands were visualized by autoradiography and quantitated by densitometry using the Article Title: 3',4'-dimethoxyflavone as an aryl hydrocarbon receptor antagonist in human breast cancer cells. Article Snippet: Protein-DNA complexes were resolved on a 5– 6% polyacrylamide gel (acrylamide:bisacrylamide ration, 30:0.8) and run in 1X TBE buffer (0.9 M Tris, 0.09 boric acid, 2 mM EDTA, pH 3.8) at 110 V. Bound complexes were visualized by autoradiography and quantitated by densitometry using the Article Title: Estrogen-induced retinoic acid receptor alpha 1 gene expression: role of estrogen receptor-Sp1 complex. Article Snippet: The following procedure was used for ER-enhanced Sp1 binding studies: 1) 200–800 fmol pure hER protein in 1 3 binding buffer containing 40 mM E2 and BSA was incubated for 15 min at 4 C; 2) 1–5 ng Sp1 protein was added to the mixture and incubated on ice for 5 min; 3) 32Plabeled oligonucleotides (50,000 cpm) were added to the reaction mixture in the presence of 0.5 mg poly d(I-C), and the mixture was incubated for an additional 15 min at 25 C. Samples were loaded onto a 5% polyacrylamide gel (acrylamide-bisacrylamide ratio, 30:0.8) and run in 1 3 TBE buffer (0.09 M Tris, 0.09 boric acid, and 2 mM EDTA, pH 8.3) at 110 V. Protein-DNA binding was visualized by autoradiography and quantitated by densitometry using the Article Title: Transcriptional activation of thymidylate synthase by 17beta-estradiol in MCF-7 human breast cancer cells. Article Snippet: Quantitation of band intensities used a |